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Following iron accumulation, the neuronal damage during reperfusion would be aggravated (Tuo et al., 2017)

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The membranes were blocked in QuickBlock Blocking Buffer for 15 min (Beyotime) and then probed with primary antibodies against fibronectin, collagen I, GFAP, -SMA, TGF1, SMAD3, phospho-Smad3 (Thr8), SMAD4, -tubulin, and -actin (Supplementary Table 2)