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The first stage is Trem2-independent and involves a reduction in homeostatic microglia checkpoint genes such as Cx3cr1, P2ry12 , and P2ry13 , along with upregulation of genes including the Trem2-signaling adaptor Tyrobp, Apoe , and B2m

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Muscle symptoms Benzodiazepines are efficient muscle relaxants and are used clinically for spastic conditions ranging from spinal cord disease or injury to the excruciating muscle spasms of tetanus or rabies

The following day, cells were treated with Accutase (Sigma) and resuspended in PBS, washed twice with Ca 2+ -free buffer (NaCl 116 mM, KCl 5.6 mM, MgCl 2 1.2 mM, NaHCO 3 5 mM, NaH 2 PO 4 1 mM, HEPES 20 mM, Glucose 1 g/L)