E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects pcnB (plasmid copy number)

pcnB (plasmid copy number)

Rapid extraction of RT-PCR-ready RNA from cultured mammalian cells

2 U/µL 10X T4 DNA Ligase Buffer High Concentration Rapid Kit 1

then induce to high copy number for DNA recovery

E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects pcnB (plasmid copy number)Table 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES