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A Dionex UltiMate 3000 506 LC System (Thermo Scientific) and a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) operated in negative mode was used for targeted measurement

It is estimated that 25% of the global population has NAFLD, a number projected to continue to rise, and as it does, it is expected that more of these cases will progress to end-stage disease states requiring liver transplantation

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