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Knocking out DDIT4 in AML12 cells significantly activated mTORC1, as shown by the increase in p-ULK1 expression, and this effect could be significantly reduced by adding Rapa (an mTOR inhibitor)

After ruling out a number of other conditions as the primary cause of sleep disturbances, including psychiatric (depression and anxiety), medical (pain), circadian (phase-delay syndrome), or other sleep disorders (restless legs syndrome/periodic limb movements, sleep-breathing disorders), the diagnosis of primary insomnia is essentially made by exclusion

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