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tumefaciens strain GV3101 carrying pYL156-RNA1 , pYL156-MEKK1 , pYL156-BAK1 / SERK4 , pYL156-CLA1 or pYL156-GFP (the vector control) was grown in LuriaBertani (LB) liquid medium containing 50 g ml 1 kanamycin and 25 g ml 1 gentamicin at 28 C for 48 h, and the culture was then transferred into fresh liquid LB medium containing 50 g ml 1 kanamycin, 25 g ml 1 gentamicin, 10 mM MES and 20 M acetosyringone for overnight incubation at 200 rpm at 28 C

in vitro , cell-based experiments

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Most of these proteins act as chemotactic factors at the site of inflammation, promoting the initial recruitment of satellite cells, neutrophils, monocytes, and, later, lymphocytes for tissue repair [52, 62,63,64,65]