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In contrast to our in vitro human model of the ischemic penumbra consisting in the combination of hiPSCs-derived neurons and rat astrocytes exposed to hypoxia and consequent low level of glucose, Bhatti and Frostig used an in vivo rat model in which the entire system of neurons, astrocytes, and blood flow is modified after permanent middle cerebral artery occlusion (pMCAo), which results in both hypoxic conditions and minimal glucose supply through major changes in collateral blood flow 36

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