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ACS Nano 4 , 36893696, doi:10.1021/nl052396o (2010)

As soon as the supernatant was centrifuged, 400 mL of the supernatant was poured into the corresponding EP tube and stored overnight at 20 C in the refrigerator before being centrifuged at 12,000 r/min for 3 min at 4 C, and approximately 2000 mL of supernatant was collected in the injection bottle liner for on-board analysis

Epithalon has demonstrated a good safety profile in experimental and limited human studies, showing minimal adverse effects when used properly in research settings