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Both enzymes, GPX7 and GPX8, are located in the endoplasmic reticulum and are necessary enzymes involved in the oxidative folding of endoplasmic reticulum proteins, and GPX8 also plays an important role in the regulation of Ca 2+ in the endoplasmic reticulum

In the absence of GRX, pre-oxidized roGFP2-iL responds only slowly to injection of 2 mM GSH while the GRX1-roGFP2-iL fusion protein shows a dramatically increased reduction rate

This suggests flexibility of the linker, which is consistent with the results of MS studies and indicates that the linker is less strained compared to compound 7a

Any references to biological activity or potential mechanisms are derived solely from publicly available scientific literature and independent laboratory research contexts.