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CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence
Transformation efficiency of >1 x 107 cfu/µg of pUC19
coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene
without IPTG induction
Ribonuclease R (RNase R), 250 U BAC CircLigase II ssDNA Ligase ligatesA 3 to 5 exoribonuclease that digests all linear RNAs except lariat or circular RNA structures. Specific: Digests all linear RNAs but does not digest lariat or circular RNA structures, or doublestranded RNA with 3' overhangs shorter than seven nucleotides Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Valuable: Use the unique properties of this exoribonuclease to study alternative splicing and gene expression Ribonuclease R