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and Mary Margaret Clausen Distinguished Professor of Metabolism and Endocrinology and Director of the Hormone Research Institute in the Diabetes Center at University of California San Francisco

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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)

As a part of the study, once the peptide was delivered, the time spent by the rats in "open arms" state in the maze was monitored