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Endogenous glucocorticoid secretion follows a conserved circadian pattern across species, with diurnal organisms (e.g., humans) showing peak concentrations during active phases (morning) and nocturnal species (e.g., rodents) displaying maximal levels during their active period (evening) (126).In humans, peak circadian cortisol secretion (approximately 06:0010:00h) (127) temporally coincides with maximal expression of tight junction proteins (occludin, claudin-5), and optimal BBB integrity

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