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Among them, Mycolicibacterium neoaurum strain EGT24E with overexpression of inherent EGT biosynthetic genes ( egtABCDE ) and L-histidine biosynthetic genes ( hisG and hisC ) as well as deletion of putative EGT-degrading enzyme (EGTase) gene produced EGT at 1.56 0.27 g/L in 216 h of fed-batch cultivation with constant supplementation of the mixture of L-histidine (15 g/L), L-methionine (30 g/L), ammonium sulfate (8 g/L), and sodium thiosulfate (8 g/L) between 48 and 120 h of cultivation

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