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By curbing mitochondrial ROS generation at complexes I and II, riboflavin disrupts the vicious oxidative cycle, leading to diminished lipid peroxidation, preserved mitochondrial integrity, and reduced initiation of cell death pathwaysa convergent neuroprotective mechanism across diverse neuropathological contexts (35, 65)

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The relative mitochondrial membrane potential was determined as the ratio of green to red MFI 29,30 using ImageJ 1.45s software (National Institutes of Health, Bethesda, USA)