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Monitoring the effluent at 220 nm, chromatography (flow rate: 1.0 mL/min) was started with a mixture of H 2 O/MeOH (40/60, v/v), the MeOH content was kept constant for 1 min at 60%, increased within 20 min to 80%, increased within 5 min to 86%, increased within 10 min to 90%, increased within 1 min to 100%, kept constant for 3 min, decreased within 1 min to 60% MeOH, followed by re-equilibration for 4 min

Jain S, Su YH, Su YP, McCloud S, Xue R, Lee TJ, Lin SC, Lin SY, Song W, Steffen JD, Hu CT

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Consulting with a healthcare professional can provide personalized guidance on incorporating B-Complex Vitamin IV therapy as part of an individualized approach to healthy aging, taking into consideration specific needs and goals