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Uncontrolled NSP activity contributes to lung damage in multiple acute and chronic inflammatory lung diseases, including acute lung injury, acute respiratory distress syndrome, chronic obstructive pulmonary disease (COPD), alpha-1 antitrypsin deficiency (AATD), bronchiectasis, and cystic fibrosis (CF) ( Figure 2) ( Figure 2 The requirement to maintain an adequate immune response to bacterial infection is a key consideration in developing treatments for neutrophil-mediated diseases

The slides were washed three times with PBS, followed by fixation in 500 L of 4% paraformaldehyde for 15 min at room temperature

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doi: 10.1186/1750-1172-1-40