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For whole-cell recordings, slices were transferred to a slice chamber (Warner Instruments) and continuously perfused with oxygenated standard ACSF solution at 32 C

Researchers storing 5-Amino-1MQ for several months or longer may consider deep-freeze storage at approximately -80C (-112F) when consistent long-term preservation is required

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Such a combined screening strategy could provide a better understanding of the effects of anti-DMG compounds on DMG and, at the same time, elucidate the permeation characteristics and potential effects of the drugs on the BBB