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qPCR quantification graphs for detecting the 3´ amplicon and 5´ amplicon of GUSB cDNA produced by Epicentre's MMLV High Performance Reverse Transcriptase Kit and two other commercially available reverse transcriptase enzymes
such as spin-columns or even CsCl centrifugation
SK-4S & SK-5S
The enzyme is supplied with a 10X Reaction Buffer
RNA 5' Polyphosphatase, 200 U size:EACH qPCR quantification graphs for detectingDephosphorylates 5 triphosphorylated RNA, such as primary RNA transcripts, but does not desphosphorylate 5 capped mRNA Specific: Removes the and phosphates from 5 triphosphorylated and the phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5 capped RNA Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Flexible: Use for various applications such as 5 RNA ligation tagging using T4 RNA Ligase,
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