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Collapse assay Cos7 cells were plated (1 10 5 cells/ml) onto glass coverslips, and 24 h later, cells were transiently transfected with plasmids encoding for empty vector (pmCherry) or co-transfected with myc-PlexinB1 together with pmCherry-RanWT, pmCherry-RanQ69L, pmCherry-RanT24N or pmCherry using Lipofectamine 2000 as per manufacturers instruction

they were used to contextualize and interpret primary experimental findings but were not treated as primary evidence when synthesizing mechanistic conclusions

For instance, it has been demonstrated to enhance self-renewal in hematopoietic stem cells and mitigate the immunosuppression induced by cyclophosphamide chemotherapy in mice

To disrupt OM for visualization of nozzles, cells swarming on hard agar with or without copper were scraped into CTT media in a 1.5 ml centrifuge tube