CircLigase-ssDNA Ligase, 1,000 U Chemicals Real-time PCR of HeLa RNA

Real-time PCR of HeLa RNA preparations treated with various DNases

biocompatibility and sterility

High Quality Data: High efficiency adapter ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions

RNAs with a 5´-diphosphorylated end are also converted to 5´-monophosphorylated RNA by RNA 5´ Polyphosphatase:

CircLigase-ssDNA Ligase, 1,000 U Chemicals Real-time PCR of HeLa RNAThermostable ligase that catalyzes the intramolecular ligation (i. e. circularization) of ssDNA templates Intramolecular ligation of single stranded DNA ends containing 5' phosphates and 3' hydroxyl groups ATP dependent, catalytic enzyme Circularizes ssDNA >30 bases long Compatible with high temperature ligation reactions due to thermostability of CircLigase enzyme Applications Production of ssDNA templates for rolling circle replication or rolling