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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s

After blocking with 5% BSA-TBST (Sigma), the membranes were incubated with the following primary antibodies from Abcam at 4C overnight: ER (1:1,000, ab3575), ER (1:1,000, ab3576), Ggt1 (1:1,000, ab175384), and Anpep (1:1,000, ab108310)

Clinical pregnancy rate was 33% for the CoQ10 group and 26.7% for the control group

Ingredients & Core Mechanism 1.-ingredients-and-core-mechanism Cinderella IV is powered by alpha-lipoic acid (ALA) , one of the strongest natural antioxidants