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1 Introduction Melatonin, a hormone produced by the pineal gland, has garnered significant attention owing to its role in reproductive system regulation ( The suprachiasmatic nucleus in the anterior hypothalamus governs the biological clock, maintains circadian rhythms, and synchronizes physiological processes with the natural lightdark cycle ( Melatonin regulates circadian rhythms and significantly influences various aspects of fertility, including the timing of ovulation, sperm production, and embryo implantation ( The potential role of melatonin in ART has recently garnered significant interest