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Without disrupting the core microbial network that maintains rumen functional stability, RMT promoted the growth of fiber-degrading taxa such as Ruminococcaceae and Saccharofermentans , thereby accelerating the recovery of fermentation function and ruminal homeostasis 51

The disulfide bond between Pt GSTF8 residue C13 and GSH was reduced on-column with 20 column volumes of rPPS buffer (20 mM potassium phosphate, 200 mM NaCl, 10 mM DTT, pH 7.4), at a flow rate of 5 ml min 1 and the column was then washed with 40 column volumes of PPS buffer at a flow rate of 5 ml min 1 to remove free GSH

These characteristics necessitate comprehensive assessment of gene flow risks to wild populations ( 7.6 Challenges for scaling up transformation protocols Scaling up transformation protocols from the laboratory to large-scale breeding applications faces multiple challenges (Joshi et al., 2018)

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