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and BAC vector and clone preparations
trfA gene under tight control of an inducible promoter for copy-number control of CopyControl clones and clones retrofitted with the EZ-Tn5<oriV/KAN-2> Transposon
End-point RT-PCR of different regions of a 14 kb message using HeLa cell extract with the QuickExtract™ RNA Extraction Kit
Lucigen recommends incubating plates for 32-40 hours before picking colonies for future experiments
E. cloni-10G Chemically Competent Cells (Subcloning Grade) TransforMax EPI300 and BAC vector and cloneE. cloni 10G Chemically Competent Cells Direct replacements for standard cloning strains (e. g., DH5, DH10B, JM109, TOP10, XL1 Blue, etc.) Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue white screening capable. Available in a range of transformation efficiencies (1 106 to 1 x 109cfu g). Convenient packaging options. High efficiency cells result in high colony counts Endorsed by SGI DNA for Gibson
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