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Proteomics approaches have been employed to detect oxidative modifications in sperm proteins

Ltd., Zhejiang, China), and hepatic hydroxyproline (HYP) content was measured using an HYP assay kit (lot number: 20180630, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), as previously described ( Histological Examination and Assessment Fixed liver tissue was dehydrated and embedded using a tissue processor (Leica ASP300) and a paraffin embedding station (Leica EG1160)

The core strategies include the following (Figure 5): (1) Supplement with glucosamine sulfate (1,500 mg/day) and chondroitin sulfate (8001200 mg/day) as precursors of cartilage matrix to promote cartilage repair

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