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Vigorous shaking introduces air bubbles and can damage the peptide through mechanical agitation

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The ventricular tissue was subjected to 5 rounds of enzymatic digestion using 0.05% pancreatin (Sigma-Aldrich) and 84 U/ml collagenase (Worthington)

Laboratory studies suggest it may help repair tendons, muscles, and even certain nervous system tissues, although more formal clinical research is needed to confirm these benefits conclusively