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For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C

Treatment is relaxing and administered in a comfortable setting while the IV drip delivers the necessary components directly into the bloodstream

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However, there were differences in chemical composition of earthworm casts between limed and unlimed soils determined using 13 C NMR spectroscopy