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10.1681/asn.2020091373 55 KimuraT.IsakaY.YoshimoriT
In controlled laboratory conditions, it may be included in experimental studies examining peptide stability, metal coordination, and molecular interaction behavior
A single colony was inoculated in Luria-Bertani medium (20 ml) with kanamycin (25 mg l-1) and cultured at 37 C overnight
Mechanisms of action Preclinical studies document: Anxiolytic effect without sedation in classical tests (elevated plus maze, open field, light-dark box) GABAergic system modulation : allosteric potentiation of GABA-A observed ex vivo Enkephalin increase (endogenous opioid peptide) and serotonergic modulation Immunomodulatory effects inherited from tuftsin: macrophage activation, T-helper modulation Behavioural models Anxiolysis is measured via time spent in open arms of the elevated plus maze (EPM), visit ratio of central zones of open field , and exit latency in light-dark box
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