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The 10dilution buffer (10% Triton x-100, 1 M NaCl, 1% Na-Deoxycholate, 5% N-Lauroylsarcosine, 5 mM EGTA) was added to the lysate, samples were centrifuged at maximum speed at 4 C for 20 min, and the following antibodies were added: -GFP (Santa Cruz, sc-9996, 5 g per sample), -BRD4 (Fortis, A700-004, 5 g per sample), -ASXL1 (made inhouse, 40 L anti-sera per sample), or -BAP1 (made inhouse, 40 L anti-sera per sample)

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Add the following selenium-rich foods to your grocery list (dietary restriction permitting!): brazil nuts (1 nut = 100% of the selenium you need for the day, so dont eat too many in one sitting!) spinach yellow-fin tuna sunflower seeds halibut baked beans turkey chicken 6

A nasal spray bottle does, and your diluent choice has to account for that from the start