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Cultured mammalian cells were treated with menadione or BSO: menadione increases oxidation of glutathione, while BSO inhibits synthesis of cellular GSH

10.1080/00207454.2017.1327857 Int J Neurosci

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Glutathione is synthesized in a two-step process, in which the first and rate-limiting enzyme -glutamylcysteine synthetase (ECS or GSH1) converts glutamate and cysteine to EC, then glutathione synthetase (GSH2) forms glutathione by the ligation of an additional glycine to the intermediate 42,43 (Fig