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Behavioral testing was followed by biochemical analyses on colon, faces, serum, cerebellum, and prefrontal cortex, assessing tryptophan metabolites, serotonin (5-HT) pathway intermediates, neurotransmitters (GABA, glutamate, dopamine, acetylcholine) via UPLC, UPLC-TQD, and ELISA, alongside gut microbiota profiling through 16S r RNA sequencing

ChaC1, in contrast, is only present in higher eukaryotes and is expressed downstream of the Integrated stress response, under different stress and pathophysiological conditions

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It is considered a conditionally essential aminoacid as, under certain conditions (hereditary disorders of the synthesis processes, hepatic insufficiency, aciduria, intense physical activity), its lack in the diet causes a state of deficiency