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FPLC purification of GST-tagged proteins under native conditions VI

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doi: 10.1136/annrheumdis-2013-204273 7 DalbethNPhipps-GreenAFramptonCNeogiTTaylorWJMerrimanTR

Chain elongation of the intermediate is mediated by a condensation (C) domain catalyzing the nucleophilic attack of the downstream PCP-bound acceptor monomer on the activated thioester of the upstream PCP-bound donor monomer 13 , thus giving rise to the dipeptide intermediate D- O -Leu-D-Ala- S -PCP