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The animal protocol was approved by the Animal Care and Use Committee of the University of Colorado, Denver

Following each experiment, the cells were washed once with PBS (LPS solution CBP007B) and fixed with 10% formalin solution (Sigma HT501128-4L) for 10 min at room temperature

IP: Conceptualization, Data curation, Investigation, Supervision, Validation, Visualization, Writing original draft

This also reflects the advantage of utilizing mustard oils against insect pests as they are easy and economical to produce as compared to AITC which is a highly costly and purified metabolic product synthesized industrially, though practical application faces significant formulation challenges including volatility, oxidation susceptibility, and the need for sophisticated stabilization technologies that could substantially increase production costs