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The structure of the GPX4 monomer lacks an internal extension of 20 amino acids that is conserved in the tetrameric GPX enzyme, which encodes a loop around the active site to prevent the entry of complex substrates into the active site [8]

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Furthermore, the development of hemolytic anemia in Prx2 knock-out mice [72, 73, 74] and the high turnover rate of Prx2 with H 2 O 2 [5, 75] strengthened the importance of the role of Prx2 in RBC antioxidant protection