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In DSS-induced colitis following cigarette smoke exposure, the six genes with the most significant differences ( Cxcl1, Ccl2, Il10, Tgfb1, Foxp3, and Cxcl10 ), all of which are Treg signature genes, were consistently downregulated in Gpr15 / mice compared with those in Gpr15 +/+ mice under identical experimental conditions (Fig

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Leaf samples were collected and processed to extract RNA for reverse transcription quantitative real-time PCR (RT-qPCR) assays as described in Turbo DNA-free, Life Technologies)