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Nat Med 22(8):879888

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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

Validation at low concentration levels for analytes naturally present at high concentrations, such as Gln, Glu, and GSH, can be challenging, as the response from low-concentration SA may not differ significantly from the endogenous concentration, leading to inaccurate results