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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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MULTI-STRAIN PROBIOTIC 20B This nutraceutical contains 20 billion active cultures per capsule and supports the digestive tract and immune function of individuals under stress

Pharmacotherapy Treatment with medications for lumbar disc degeneration is currently focused on relieving symptoms, though novel therapies aimed at modifying disease progressionsuch as anti-cytokine and anti-fibrotic compoundsare being studied and may work effectively when combined with biological treatments like platelet-rich plasma and stem cell approaches