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Briefly, it covered: a) removal of low-quality precursors and contaminant protein groups, b) precursor intensities normalization by loessF algorithm c) protein group MaxLFQ intensities calculation and log2 transformation, and d) differential expression analysis using LIMMA statistical test

A number of mechanisms appear to contribute to the mucolytic and mucoregulatory benefits of NAC, including directly breaking disulphide bonds between the molecules forming the mucus with its thiol groups and reducing mucus production through its antioxidant effects (oxidative stress has been shown to increase mucus production)