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Nakagami et al., 2001)

Stereological brain sampling of the areas of interest was performed and brain were serially cut on a cryostat (Leica Biosystems, Germany, cat# CM3050S), mounted on Superfrost Plus slides (Thermo Fisher Scientific, Reinach, Switzerland), and cryoprotected in 50% of PB, 30% of ethylene glycol, 20% of glycerol, and subsequently stored at 20 C

Observed responses in research settings are generally reported as mild and may include: Temporary injection-site irritation Redness or warmth sensations Skin-sensitivity variability Temporary blue-tinted discoloration beneath the administration area Localized irritation depending on dosage range, concentration, administration frequency, protocol duration, and individual sensitivity Research protocols commonly investigate daily administration ranges between: 12mg daily in standard research settings Up to 3mg daily in more advanced experimental protocols GHK-Cu is frequently explored in continuous or maintenance-style research structures and does not always require strict cycling protocols in moderate-dose experimental models