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4 The 2020 American College of Rheumatology guidelines 5 conditionally recommend screening for the human leukocyte antigen (HLA)-B*58:01 allele in certain populations before starting allopurinol, as carriers of this allele have a higher risk of TEN than noncarriers

Immunoblotting Tissue samples from brain were lysed in cold RIPA buffer supplemented with phosphatase inhibitor and protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) using a FastPrep 24 (M.P