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The hallmarks of cellular senescence, e.g., the appearance of senescence-associated -galactosidase (SA--gal) and heterochromatin foci (SAHF) as well as the increased expression of cell cycle inhibitor proteins, i.e., p16, p21, and p53, are also common markers of senescent immune cells, especially in Teff cells [154,155,156]

Before applying to live cells, the cytotoxicity of TPE-DCV is evaluated by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay (Figure S13)

BTK found involved in the 19 diseases associated signaling pathways like pathway Chemotaxis_CXCL12/CXCR4-induced chemotaxis of immune cells includes BTK and JAK3, but does not include ITK, while Immune response_NF-AT in immune response involves BTK and ITK but not JAK3