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The assay is based on the cleavage of yellow tetrazolium salt to purple formazan crystals by metabolically active cells

ROS can activate MMPs (including MMP-2) and simultaneously decrease concentrations of TIMPs (including TIMP-2) and this can contribute to BBB permeability (68)

At low JA-Ile levels, the transcription of JA-responsive genes is repressed because of the accumulation of JAZ proteins, but this repressed state is de-repressed by JA-Ile levels elevated in response to environmental and developmental cues, which promote the binding of JA-Ile with JAZ in the COI1 coreceptor and initiate ubiquitination and the subsequent degradation of JAZ by proteasomes

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