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Once drawn, specimens were separated and the ethylenediaminetetraacetic acid (EDTA) tubes were placed at 4C, whereas the larger gel tubes were allowed to clot for fifteen minutes and then centrifuged at 3000 revolutions per minute (Champion F-33 Series, Ample Scientific, Norcross, GA) for fifteen minutes

Both pathways lead to the release of growth hormone, but by different molecular routes

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