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was done, followed by rinsing in PBS

The Two Structural Modifications and Their Research Significance Analogues with substitutions, deletions, or insertions at the N-terminal end of native IGF-1 were found to reduce binding to IGFBPs and increase bioavailability to IGF-1R on cells a principle exploited in the design of LR3, where the arginine substitution and N-terminal extension together confer superior IGFBP evasion compared to shorter analogues such as Arg3 IGF-1, Gly3 IGF-1, and Des(1-3) IGF-1

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Pathways such as regulation of peptidase activity, extracellular matrix organization, regulation of protein dephosphorylation, regulation of metal ion transport, and vesicle-mediated transport were enriched in all the comparisons