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Results Cystine availability regulates mTORC1 signaling and the ISR We studied the effect of cystine starvation in HepG2 cells, which do not synthesize cysteine from methionine, to exclude the confounding effect of methionine 20

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Force curves were collected by approaching the functionalized tip on different points of the substrates surfaces at the constant velocity of 50 nm/s, while the retraction velocity was varied from 50 to 4,200 nm/s, according to the selected nominal loading rates, defined as the product of the nominal cantilever spring constant ( k nom ) by the tip pulling velocity ( v ), and set in the nominal range of 184 nN/s

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