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Extrachromosomal double-strand break substrate design DNA double-strand break extrachromosomal substrates were obtained as single-strand oligonucleotides from Integrated DNA Technologies (ultramers, IDT) and annealed in 10 mM Tris, pH 7.5 supplemented with 100 mM NaCl and 0.1 mM EDTA

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10.1016/j.semcdb.2015.01.009 85 LangK