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Obesity disrupts the HPG axis and reproductive hormone balance and raises oxidative stress levels, resulting in reduced sperm concentration, motility, and viability, as well as increased numbers of abnormal sperm

In line with the previously shown lack of enzyme expression in normal lung tissue (Supplementary Fig

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The interacting proteins can then be eluted and analyzed, typically through SDS-PAGE followed by mass spectrometry or Western blotting, to identify and characterize the protein interactions