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Primary antibodies used were GAPDH (Sigma-Aldrich, #G8795, diluted 1:10,000), AKAP1 (Sigma-Aldrich, #SAB4503247, diluted 1:500), PHACTR1 (Abcam, #ab229120), Total OXPHOS Blue Native WB Antibody Cocktail (Abcam, #ab110412), HMOX1 (Santa Cruz Biotechnology, #sc-7695) and the following all from Cell Signaling: CDT1 (#8064), Thymidine Kinase 1 (#28755), Phospho-Histone H3 (Ser10, #53348), Cyclin A2 (#91500), Cyclin B1 (#12231), Cyclin E1 (#20808), Phospho-cdc2 (Tyr15, #4539), Tom20 (#42406), OPA1 (#80471), DRP1 (#8570), Phospho-DRP1 (Ser616, #4494), Phospho-DRP1 (Ser637, #6319), MFF (#84580), Mitofusin-1 (#14739), Mitofusin-2 (#11925), GPX4 (#52455), NCOA4 (#66849), KEAP1 (#8047), FTH1 (#4393) and 4F2hc/SLC3A2 (#47213)
beginners should start with lower or spaced doses under medical supervision
Revel in the luxury of Japanese technology and ingredients that promise to deliver not just whitening but also rejuvenating and anti-aging benefits
& Kirov, S
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