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The qRT-PCR was conducted in 20 L reactions containing SYBR Green Real-Time PCR Master Mix (TaKaRa, Japan) on a StepOnePlus Real-Time PCR System (Thermo Scientific, USA), with three biological replicates per treatment

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The researchers of the current study used this technique to analyze the methylation patterns in the genome from tumour samples obtained from the Guru Nanak Institute of Dental Science and Research in West Bengal, in collaboration with Prof