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Tetrahydrobiopterin (BH 4 ) is an essential cofactor for eNOS required for efficient electron transfer during the synthases catalytic cycle ( 4 keeps eNOS in a coupled state, in which it synthesizes NO rather than harmful superoxide ( 4 can be produced through two pathways: de novo synthesis , regulated by the rate-limiting enzyme guanosine triphosphate cyclohydrolase I (GTPCH), or through the salvage pathway , where dihydrobiopterin (BH 2 ) is recycled back to BH 4 by dihydrofolate reductase (DHFR) (Crabtree and Channon, 2011)

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Identification of renox, an NAD(P)H oxidase in kidney

Spirulina s medicinal activities are often presumed to be based on species